Isolation of endophytic bacteria from nutmeg plant as antibacterial agents against pathogenic bacteria

Melda Yunita, Yosephina Manse, Myllisa Citra Wulandari, Sukmawati Sukmawati, Morgan Ohiwal

Abstract


 Recently, exploration of natural antibiotic alternatives by utilizing medicinal plants is often carried out. Nutmeg plant (Myristica fragrans Houtt) is a tropical plant endemic to the Maluku Island and often used as medicine by local people. This study was aimed to isolate endophytic bacteria from the nutmeg plant and detect its potential as an antibacterial against pathogenic bacteria. The study is a descriptive observational study with a true experimental approach. Endophytic bacteria were isolated from several organs of the nutmeg plant such as leaves, leaf bones, stems, fruit flesh, and seeds and then tested for their inhibition against Staphylococcus aureus, Propionibacterium acne, and Escherichia coli. The results showed that: (1) the highest total population of endophytic bacteria was found in the seeds of nutmeg (7.5x104 CFU/ml), (2) ten isolates of endophytic bacteria were obtained with varying morphological characteristics, and (3) the detection of antibacterial activity showed that of the 10 isolates obtained, 4 isolates were able to inhibit the growth of Escherichia coli, namely isolate BEAD2, BEATD1, BEABJ 2, and BEABJ 1 with the diameter of the inhibition zones of each isolate being 3.4 mm, 3.2 mm, 1.6 mm and 1.5 mm respectively. The conclusion of this study is that 4 isolates of endophytic bacteria were detected as potential antibacterial against Escherichia coli.


Full Text:

PDF

References


Agaus, L.R., & Agaus, R.V. (2019). Manfaat kesehatan tanaman pala (myristica fragrans) (health benefits of nutmeg (Myristica fragrans). Medula, 6(3), 662–6. Retrieved from http://ojs.uho.ac.id/index.php/medula/article/download/9648/6842

Aglinia, M., Pujiyanto, S., & Wijanarka, W. (2020). Isolasi bakteri endofit bangle (zingiber cassumunar roxb.) dan uji antibakteri supernatan crude metabolit sekunder isolat potensial terhadap Staphylococcus aureus. Jurnal Akademika Biologi, 9(1), 23-31. Retrieved from https://ejournal3.undip.ac.id/index.php/biologi/article/view/27742

Arrizqiyani, T., Sumiati, S., & Meliansyah, M. (2018). Aktivitas antibakteri daging buah dan daun pala (Myristica fragrans) terhadap escherichia coli. Jurnal Vokasi Kesehatan, 4(2), 291-294. Retrieved from http://ejournal.poltekkes-pontianak.ac.id/index.php/JVK/article/download/119/pdf

Bhore, S.J., & Sathisha, G. (2010). Screening of endophytic colonizing bacteria for cytokinin-like compounds: crude cell-free broth of endophytic colonizing bacteria is unsuitable in cucumber cotyledon bioassay. World Journal of Agricultural Science, 6(4), 345–52. Retrieved from https://www.semanticscholar.org/paper/Screening-of-endophytic-colonizing-bacteria-for-of-Bhore-Sathisha

Gansareng, A., Lolo, W.A., & Pelealu, N.C.H. (2018). Aktivitas antibakteri ekstrak kulit buah pala (myristica fragrans houtt) terhadap bakteri escherichia Coli. Pharmacon, 7(3), 52–57. Retrieved from https://ejournal.unsrat.ac.id/index.php/pharmacon/article/view/20239

Harahap, I., Rahmi, V.P., & Herlina, N. (2018). Uji aktivitas antibakteri dari isolat cendawan endofit asal tumbuhan senduduk (melastoma malabathricum l.) terhadap escherichia coli dan staphylococcus aureus. Photon Jurnal Sains dan Kesehatan, 8(2), 7–12. Retrieved from https://ejurnal.umri.ac.id/index.php/photon/article/view/741

Iqlima, D., Ardiningsih, P., & Wibowo, M.A. (2017). Aktivitas antibakteri isolat bakteri endofit b2d dari batang tanaman yakon (smallanthus sonchifolius (poepp. & endl.) h. rob.) terhadap bakteri staphylococcus aureus dan salmonella thypimurium. Jurnal Kimia Khatulistiwa, 7(1), 36–43. Retrieved from https://jurnal.untan.ac.id/index.php/jkkmipa/article/view/23569

Irdawati, I., Advinda, L., & Angraini, F. (2017). Isolasi dan uji aktivitas antimikroba bakteri endofit dari daun salam (syzygium polyanthum wight). Bioscience, 1(2), 62. Retrieved from http://ejournal.unp.ac.id/index.php/bioscience/article/download/8074/6185

Kiriwenno, J.V., Yunita, M., & Latuconsina, Z.V. (2021). Perbandingan aktivitas antibakteri ekstrak daun katang-katang (ipomoea pes-capre l.) dengan minyak seith terhadap pertumbuhan staphylococcus aureus. Majalah Farmaseutik, 17(1), 122-131. Retrieved from https://jurnal.ugm.ac.id/majalahfarmaseutik/article/view/58292

Kusumawati, D.E., Pasaribu, F.H., & Bintang M. (2014). Aktivitas antibakteri isolat bakteri endofit dari tanaman miana (coleus scutellariodes [l.] benth.) terhadap staphylococcus aureus dan escherichia coli. Current Biochemistry, 1(1), 45–50. Retrieved from https://journal.ipb.ac.id/index.php/cbj/article/view/17887/pdf

Mardianti, O., Darwis, W., & Sariyanti, M. (2019). Uji efektivitas ekstrak kayu tumbuhan biau (psophocarpus sp.) terhadap bakteri salmonella typhi dan shigella dysenteriae penyebab diare. JRK Jurnal Kedokteran Raflesia, 5(1), 28–38. Retrieved from https://ejournal.unib.ac.id/index.php/jukeraflesia/article/view/8779

Marsaoli, F., Matinahoru, J.M., & Leiwakabessy, C. (2019). Isolasi, seleksi, dan uji antagonis bakteri endofit diisolasi dari salawaku (falcataria mollucana) dalam menekan pertumbuhan cendawan patogen cercospora spp. Agrologia, 8(2). Retrieved from https://ojs.unpatti.ac.id/index.php/agrologia/article/view/1009

Mubarokhah, L., Wijanarka, W., & Rukmi, M.I. (2020). Isolasi dan penapisan bakteri proteolitik endofit tanaman pepaya (Carica papaya L .). J Trop Bio, 3(2), 99–104. Retrieved from https://ejournal2.undip.ac.id/index.php/niche/article/view/9571

Mutsaqof, A.A.N., Wiharto, W., & Suryani, E. (2015). Sistem pakar untuk mendiagnosis penyakit infeksi menggunakan forward chaining. Jurnal Teknologi dan Informasi ITSmart, 4(1), 43-47. Retrieved from https://jurnal.uns.ac.id/itsmart/article/view/1758

Nuryah, A., Yuniarti, N., & Puspitasari, I. (2019). Prevalensi dan evaluasi kesesuaian penggunaan antibiotik pada pasien dengan infeksi methicillin resistant staphylococcus aureus di rsup dr. soeradji tirtonegoro klaten. Majalah Farm,aseutik, 15(2), 123-128. Retrieved from https://jurnal.ugm.ac.id/majalahfarmaseutik/article/view/47911

Purwanto, U.M.S.P., Pasaribu, F.H., & Bintang, M. (2017). Isolasi bakteri endofit dari tanaman sirih hijau (piper betle l.) dan potensinya sebagai penghasil senyawa antibakteri. Current Biochemistry, 1(1), 51–57. Retrieved from https://journal.ipb.ac.id/index.php/cbj/article/view/17890

Reppi, N.B., Mambo, C., & Wuisan, J. (2016). Uji efek antibakteri ekstrak kulit kayu manis (Cinnamomum burmannii ) terhadap Escherichia coli dan Streptococcus pyogenes. J e-Biomedik, 4(1). Retrieved from https://ejournal.unsrat.ac.id/index.php/ebiomedik/article/view/12204

Rumopa, P.M.E, Awaloei, H., & Mambo, C. (2016). Uji daya hambat ekstrak biji pala (myristicae fragrans) terhadap pertumbuhan bakteri staphylococcus aureus dan streptococcus pyogenes. Jurnal e-Biomedik, 4(2). Retrieved from https://ejournal.unsrat.ac.id/index.php/ebiomedik/article/view/14655

Seta, I.S., & Rizka, R. (2015). Pola kepekaan bakteri penyebab infeksi saluran kemih pada anak terhadap antimikroba. Majalah Kedokteran Sriwijaya, 47(2), 85–90. Retrieved from https://ejournal.unsri.ac.id/index.php/mks/article/view/2748

Sumampouw, O.J. (2018). Uji sensitivitas antibiotik terhadap bakteri escherichia coli penyebab diare balita di kota manado. Journal of Current Pharmaceutical Science, 2(1), 104-110. Retrieved from https://journal.umbjm.ac.id/index.php/jcps/article/download/171/111/

Yarza, H.L., Yanwirasti, Y., & Irawati L. (2015). Hubungan tingkat pengetahuan dan sikap dengan penggunaan antibiotik tanpa resep dokter. Jurnal Kesehatan Andalas, 4(1), 151–156. Retrieved from http://jurnal.fk.unand.ac.id/index.php/jka/article/view/214

Yunita, M., & Sukmawati S. (2021). Edukasi bahaya resistensi bakteri akibat penggunaan antibiotik yang tidak rasional kepada masyarakat desa air salobar. Indonesia Berdaya, 2(1), 1-6. Retrieved from https://ukinstitute.org/journals/ib/article/view/211

Yunita, M., Mubarik, N.R., & Solihin, D.D. (2016). Isolation and identification of chitinolytic bacteria as biocontrol agent of pathogenic fungi on gold silkworm cocoon Cricula trifenestrata. Malaysian Journal of Microbiology, 12(1), 69-75. Retrieved from https://www.semanticscholar.org/paper/Isolation-and-Identification-of-Chitinolytic-as-of-Yunita-Mubarik

Zahrah, H., Mustika, A., &Debora, K. (2019). Aktivitas antibakteri dan perubahan morfologi dari propionibacterium acnes setelah pemberian ekstrak curcuma xanthorrhiza. J Biosains Pascasarjana, 20(3), 162-168. Retrieved from https://e-journal.unair.ac.id/BIOPASCA/article/download/14073/7891




DOI: https://doi.org/10.31932/jpbio.v7i1.1522

Article Metrics

Abstract view : 59 times
PDF - 27 times

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.