Antibacterial potential of leaf and fruit of citrus extract (Citrus aurantifolia) against klebsiella oxytoca

Zuraidah Zuraidah, Widiasari Widiasari, Wati Oviana

Abstract


Antimicrobial agents play a crucial role in decreasing the impact of infectious diseases worldwide. Many medicinal plants have been identified as rich sources of natural antimicrobial compounds that can be used as an alternative to traditional treatments for bacterial infections. Indonesia, particularly in the Aceh region, has a warm and humid climate that supports a diverse range of plants that have medicinal properties. One example is the lime (Citrus aurantifolia) which can be used for medicinal purposes.This study aimed to determine the inhibitory effects of leaves and fruit extracts of lime on the growth of Klebsiella oxytoca.The method used was an experimental method with a post-test only control group design. The results showed that both types of extracts contain flavonoid, alkaloid, and saponin compounds. However, triterpenoids were only found in the fruit extract, and steroids were only found in the leaf extract. The antimicrobial test of lime fruit and leaf extracts against Klebsiellaoxytoca bacteria showed that both types of extracts were able to inhibit the growth of the test bacteria at all concentrations.


Full Text:

PDF

References


Afroja, S., Falgunee, F.N., Jahan, M.M., & Akanda, K.M. (2018). Antibacterial activity of different citrus fruits antibacterial activity of different citrus fruits. Specialty Journal of Medical Research and Health Science, 2(2), 25–32. Retrieved from https://sciarena.com/storage/models/article/z1kmvRCn35k6j6CF10wBBAgOei9gTLPIHp8sUZqbXm5zEQMl09H7Vzen6RTc/antibacterial-activity-of-different-citrus-fruits.pdf

Apraj, V., Thakur, N., Bhagwat, A., Mallya, R., & Sawant, L. (2011). Pharmacognostic and phytochemical evaluation of citrus aurantifolia ( christm ) swingle PEEL. Pharmacognosy Journal, 3(26), 70–76. Retrieved from https://doi.org/10.5530/pj.2011.26.12

Chakraborty, S., Mohsina, K., Sarker, P.K., Zahangir Alam, M., Abdul Karim, M.I., & Abu Sayem, S.M. (2016). Prevalence, antibiotic susceptibility profiles and ESBL production in klebsiella pneumoniae and klebsiella oxytoca among hospitalized patients. Periodicum Biologorum, 118(1), 53–58. Retrieved from https://doi.org/10.18054/pb.2016.118.1.3160

Echeverri, L.M., & Catano, J.C. (2010). Klebsiella pneumoniae as nosocomial pathogens: epidemiology and resistance. Atreia, 23(3), 240–249. Retrieved from https://doi.org/10.1128/cmr.11.4.589

Enejoh, O.S., Ogunyemi, I.O., Bala, M.S., Oruene, I.S., Suleiman, M.M., & Ambali, S.F. (2015). Ethnomedical importance of citrus aurantifolia (christm) swingle. The Pharma Innovation Journal, 4(8), 1–6. Retrieved from www.thepharmajournal.com

Fratianni, F., Cozzolino, A., & Feo, V. De. (2019). Polyphenols, antioxidant, antibacterial, and biofilm inhibitory activities of peel and pulp of citrus medica l., citrus bergamia, and citrus medica cv. salò cultivated in southern italy florinda. Molecules, 24(4577), 1–14. Retrieved from https://doi.org/doi:10.3390/molecules24244577

Izah, S.C. (2018). Some determinant factors of antimicrobial susceptibility pattern of plant extracts. Research and Review Insights, 2(3), 1–4. Retrieved from https://doi.org/10.15761/RRI.1000139

Jayaprakasha, G.K., Mandadi, K.K., Poulose, S.M., Jadegoud, Y., Nagana Gowda, G.A., & Patil, B.S. (2008). Novel triterpenoid from Citrus aurantium L. possesses chemopreventive properties against human colon cancer cells. Bioorganic and Medicinal Chemistry, 16(11), 5939–5951. Retrieved from https://doi.org/10.1016/j.bmc.2008.04.063

Loizzo, M.R., Tundis, R., Bonesi, M., Menichini, F., De Luca, D., Colica, C., & Menichini, F. (2012). Evaluation of citrus aurantifolia peel and leaves extracts for their chemical composition, antioxidant and anti-cholinesterase activities. Journal of the Science of Food and Agriculture, 92(15), 2960–2967. Retrieved from https://doi.org/10.1002/jsfa.5708

Manandhar, S., Luitel, S., & Dahal, R.K. (2019). In Vitro antimicrobial activity of some medicinal plants against human pathogenic bacteria. Journal of Tropical Medicine, 1(1), 1–5. https://doi.org/10.1155/2019/1895340

Minami, J., Okabe, A., Shiode, J., & Hayashi, H. (1989). Production of a unique cytotoxin by Klebsiella oxytoca. Microbial Pathogenesis, 7(3), 203–211. Retrieved from https://doi.org/10.1016/0882-4010(89)90056-9

Mubarak, Z., & Soraya, C. (2018). The acid tolerance response and pH adaptation of Enterococcus faecalis in extract of lime Citrus aurantiifolia from Aceh Indonesia. F1000Research, 7(0), 287. Retrieved from https://doi.org/10.12688/f1000research.13990.1

Neog, N., Phukan, U., Puzari, M., Sharma, M., & Chetia, P. (2021). Klebsiella oxytoca and Emerging Nosocomial Infections. Current Microbiology, 78(4), 1115–1123. Retrieved from https://doi.org/10.1007/s00284-021-02402-2

Okwu, D.E. (2005). Phytochemicals, vitamins, and mineral contents of two nigerian medicinal plants. International Journal of Molecular Medicine and Advance Sciences. Retrieved from https://www.medwelljournals.com/abstract/?doi=ijmmas.2005.375.381

Rahman, S., Parvez, A.K., Islam, R., & Khan, M.H. (2011). Antibacterial activity of natural spices on multiple drug resistant Escherichia coli isolated from drinking water , Bangladesh. Annals of Clinical Microbiology and Antimicrobials, 10(10), 1–4. Retrieved from https://doi.org/10.1186/1476-0711-10-10

Ramona, A., Erika, B., Anna, C., Maria, M., & Sobarzo-s, D.E. (2016). Phytochemicals for human disease: An update on plant-derived compounds antibacterial activity. Microbiological Research, 196, 44–68. Retrieved from https://doi.org/10.1016/j.micres.2016.12.003

Sandoval-montemayor, N.E., García, A., Elizondo-treviño, E., Garza-gonzález, E., Alvarez, L., & Camacho-corona, M.R. (2012). Chemical composition of hexane extract of citrus aurantifolia and anti-mycobacterium tuberculosis activity of some of its constituents. Molecules, 17, 11173–11184. Retrieved from https://doi.org/10.3390/molecules170911173

Tavallali, H., Bahmanzadegan, A., Rowshan, V., & Tavallali, V. (2021). Essential oil composition, antioxidant activity, phenolic compounds, total phenolic and flavonoid contents from pomace of citrus aurantifolia. Journal of Medicinal Plants and By-Products, 1, 103–116. Retrieved from https://doi.org/10.22092/jmpb.2020.341476.1175

Wardani, R., Jekti, D.S.D., & Sedijani, P. (2018). Uji aktivitas antibakteri ekstrak kulit buah jeruk nipis (citrus aurantifolia swingle) terhadap pertumbuhan bakteri isolat klinis. Jurnal Penelitian Pendidikan IPA, 5(1). Retrieved from https://doi.org/10.29303/jppipa.v5i1.101

Yang, J., Long, H., Hu, Y., Feng, Y., McNally, A., & Zong, Z. (2022). Klebsiella oxytoca complex: Update on taxonomy, antimicrobial resistance, and virulence. Clinical Microbiology Reviews, 35(1). Retrieved from https://doi.org/10.1128/CMR.00006-21




DOI: https://doi.org/10.31932/jpbio.v8i1.2261

Article Metrics

Abstract view : 230 times
PDF - 157 times

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.