The use of alternative coloring variations to mitotic observations of shallots using the squash method

Ni Luh Tirtasari, Luthfi Hanum Saputri, Nur Kusuma Dewi, Siswanti Siswanti

Abstract


Practicum cannot be carried out due to limited facilities and infrastructure such as dyes for observation of mitotic cell chromosomes in onions. Alternative dyes that can be obtained from nature can be explored. The purpose of this study was to obtain preparation of the mitotic division process in various onion root cells using alternative dyes using dyes from dragon fruit peel and turi flower. In obtaining the right preparation using the squash method. Data were obtained using observation sheets that observed each variation of the material. The instrument used in this study is a valid observation sheet. The results showed that the coloring of the preparation using dragon fruit skin and turi flowers was successful and clear coloring results were obtained. Coloring in practicum activities using turi flowers is better than dragon fruit coloring. Natural dyes from dragon fruit peel and turi flower can be used as alternative dyes in the squash method to observe onion root mitosis, replacing synthetic dyes. 

Full Text:

PDF

References


Abidin, A. Z., Djoko Budiono, J., & Isnawati. (2014). Studi indeks mitosis bawang untuk pembuatan media pembelajaran preparat mitosis. BioEdu, 3(3), 571-579. Retrieved from https://ejournal.unesa.ac.id/index.php/bioedu/article/view/9589

Anam, C. (2016). Studi pemanfaatan potensi pigmen kulit buah naga merah (Hylocereus polyrhizus) sebagai pewarna alami preparat section tumbuhan sirsak (Annona muricata) dikembangkan sebagai bahan ajar biologi. Thesis. Malang: Universitas Muhammadiyah Malang.

Aryadini, S. N. R., Putri, V. A., & Wahyuni, S. (2003). Analisis perbandingan preparat mikroskopis pembuluh trakea pada Epipremnum aureum menggunakan pewarnaan safranin dan methylen blue. Prosiding Seminar Nasional Pendidikan Biologi, 649–654.

Aziz, I. R. (2019). Kromosom tumbuhan sebagai marka genetik. Teknosains: Media Informasi Sains Dan Teknologi, 13(2). Retrieved from https://journal.uin-alauddin.ac.id/index.php/teknosains/article/view/9638

Bisri, C. (2014). Ekstrak kelopak bunga rosella (Hibiscus sabdariffa l.) sebagai pewarnaan alternatif alami preparat section tanaman cabe merah besar (Capsicum annuum l. Thesis: Univeristas Muhammadiyah Malang.

Hidayah, T., Pratotjo, W., & Widiarti, N. (2014). Uji stabilitas pigmen dan antioksidan ekstrak zat warna alami kulit buah naga. Inonesian Journal of Chemical Science, 3(2), 135–140. Retrieved from https://journal.unnes.ac.id/sju/ijcs/article/view/3500

Latifa, R. (2015). Peran biologi dan pendidikan biologi dalam menyiapkan generasi unggul dan berdaya saing global. Prosiding Seminar Nasional Biologi

McIntosh, J. R. (2016). Mitosis. Cold Spring Harbor Perspectives in Biology, 8(9). Retrieved from https://cshperspectives.cshlp.org/content/8/9/a023218.short

Muslim, N. Y. A., & Rosidah, A. (2023). Validitas ekstrak asam sitrat kulit buah naga merah (hylocereus polyrhizus) sebagai pewarna sel hepatosit pada preparat jaringan organ hepar tikus Rattus Norvegicus L. Jurnal Kedokteran Komunitas (ournal of Community Medicine, 11.(2). https://jim.unisma.ac.id/index.php/jkkfk/article/view/23068

Perilli, S., Di Mambro, R., & Sabatini, S. (2012). Growth and development of the root apical meristem. Current Opinion in Plant Biology, 15(1), 17–23. Retrieved from https://doi.org/10.1016/j.pbi.2011.10.006

Permatasari, R., & Suriani, E. (2022). Potensi buah naga merah (Hylocereus costaricensis) sebagai pewarnaan alternatif pengganti eosin pada pewarnaan papanicolaou terhadap sediaan apusan epitel mulut ayam. JUKEJ: Jurnal Kesehatan Jompa, 1(2830), 9. Retrieved from https://doi.org/10.55784/jkj.Vol1.Iss1.103

Saati, E. A. (2008). Pengaruh jenis pelarut pada proses ekstraksi terhadap kualitas pigmen bunga turi (Sesbania grandiflora (L) Pers). Eprints Umm.

Said, F., Rahmawati, I., & Triwiyatini, T. (2021). Gel ekstrak buah naga super merah (Hylocereus Costaricensis) dan ubi jalar ungu sebagai alternatif pewarna (Discloting Solution) alami plak gigi. An-Nadaa: Jurnal Kesehatan Masyarakat (e-Journal), 8(2), 148–151. Retrieved from https://ojs.uniska-bjm.ac.id/index.php/ANN/article/view/5754

Sharma, A. (2020). Chromosome techniques. CRC Press. Retrieved from https://doi.org/10.1201/9780203743164

Simanjuntak, L., & Sinaga, C. (2014). Ekstraksi pigmen antosianin dari kulit buah naga merah (Hylocereus polyrhizus). Jurnal Teknik Kimia USU 3(2). Retrieved from https://download.garuda.kemdikbud.go.id/article.php?article=2805901&val=24909&title=EKSTRAKSI%20PIGMEN%20ANTOSIANIN%20DARI%20KULIT%20BUAH%20NAGA%20MERAH%20Hylocereus%20polyrhizus

Susliana, D., & Wahyuni, S. (2019). Pengembangan media pembelajaran scrapbook untuk meningkatkan pemahaman materi. Unnes Physic Education Journal, 8(3), 220–227. Retrieved from https://journal.unnes.ac.id/sju/upej/article/view/35618

Virgianti, D. P., & Luciana, C. (2017). Penggunaan ekstrak kombinasi angkak dan daun jati sebagai pewarna penutup pada pewarnaan gram. Jurnal Kesehatan Bakti Tunas Husada, 17. Retrieved from https://ejurnal.universitas-bth.ac.id/index.php/P3M_JKBTH/article/view/191

Wahyuni, I. N., & Sabban, I. F. (2022). Efektivitas hasil pewarnaan sediaan feses dengan ekstrak buah naga merah (Hylocereus Polyrhizus) sebagai pengganti eosin. Jurnal Wiyata: Penelitian Sains dan Kesehatan, 9(2), 115–121. Retrieved from https://ojs.iik.ac.id/index.php/wiyata/article/view/620

Wahyuni, S. (2015). Identifikasi preparat gosok tulang (Bone) berdasarkan teknik pewarnaan. Prosiding Seminar Nasional Pendidikan Biologi. Retrieved from https://biology.umm.ac.id/files/file/657-666%20Sri%20Wahyuni.pdf

Yang, X. (2009). Observation of mitosis and meiosis in rice cells by simple squash method. Agricultural Science & Technology-Hunan, 10(5), 96–99. Retrieved from https://www.cabidigitallibrary.org/doi/full/10.5555/20093333229




DOI: https://doi.org/10.31932/jpbio.v9i1.3053

Article Metrics

Abstract view : 385 times
PDF - 198 times

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.